【Aim】 By comparing the differences in sensitivity and physiological and biochemical responses of the invasive species Aleyrodes proletella and Aleyrodes sp. to different insecticides, we analyzed the mechanism of the sensitivity differences, and provided a basis for the development of differentiated prevention and control strategies. 【Method】 The indoor virulence of eight insecticides against two species of whitefly adults was determined by leaf dipping method, and the field efficacy was evaluated by field efficacy test, and the contents of protective enzymes, detoxification enzymes and oxidative stress indexes of the two species of whitefly adults were examined after the treatment of sub-lethal concentration (LC₂₅). 【Result】 The adult whiteflies of the two species differed in their sensitivity to the eight insecticides,A. proletella was more sensitive to bifenthrin, abamectin, and bromoxynil, with an LC₅₀＜2.31 mg·L^-1, whileAleyrodessp. whiteflies were more sensitive to bifenthrin and abamectin, with an LC₅₀＜3.21 mg·L^-1; the sub-lethal concentrations of the different agents (LC₂₅) treatments significantly altered the enzyme activity response patterns of the two whitefly species. Under insecticide treatment,A. proletella SOD activities increased by 1.86%, POD activities increased by 2.12%-16.89%, and GST content rose 4.75%-22.74%, while Aleyrodes sp. formed the main detoxification pathway through the up-regulation of CarE activity by 0.99%-23.97%, and the CAT content rose 2.74%-9.61%. The results of field efficacy showed that 7 d after the drug, 70% thiamethoxam and 10% acetonitrile suspension reached more than 90% against A. proletella adults, which was significantly higher than that of 5% methylamino abamectin benzoate microemulsion and 10% acetonitrile suspension; the efficacy of 10% avermectin against Aleyrodes sp. adults was 89.63%, which was significantly higher than that of 5% methamino abamectin benzoate microemulsion and 10% acetonitrile suspension; and the efficacy of 10% abamectinsuspension concentrate Aleyrodes sp. adults was 89.63%, which was significantly higher than that of 5% methamino abamectin benzoate microemulsion. It was significantly higher than that of 5% methylamino abamectin benzoate microemulsion and 10% lufenuron suspension. 【Conclusion】 As shown in the indoor toxicity determination and field efficacy test, the toxicity of eight different insecticides to A. proletella was stronger than that to Aleyrodes sp. Under insecticide stress, A. proletella developed different sensitivities to insecticides through different responses to physiological enzyme contents inAleyrodessp. The best efficacy of 70% thiamethoxam water-dispersible granules against A. proletella was 94.02% at 7 d post-dose, and the best efficacy of 5% methamido-abamectin benzoate microemulsion against Aleyrodes sp. was 89.93% at 14 days post-dose, and the best efficacy of 70% thiamethoxam water-dispersible granules against A. proletella with 81.85% and 10% abamectin suspension with 80.96%. Therefore, it can be recommended to apply 70% thiamethoxam aqueous dispersible granules, 5% methamido-abamectin benzoate microemulsion and 10% abamectin suspension alternately in the field for the control and prevention of A. proletella and Aleyrodes sp.