【Aim】 This study clarifies the effects of temperature on the growth and development of Lobesia botrana and explores the effects of short-term high temperature stress on the duration and survival rate of insects at various stages, aiming to provide a basis for the prediction and sustainable control of the population dynamics of this insect species. 【Method】 The effects of temperature on the survival rate, adult mating, and spawning of the larvae and pupae of L. botrana were studied under five constant temperature conditions (24, 27, 30, 33, and 36 ℃). In addition, after treatment at 5 high temperature gradients (33, 36, 39, 42, and 45 ℃) for 30, 60, and 90 min, the developmental duration and survival of L. botrana were studied under high temperature stress. 【Result】 The survival rate of larvae and pupae gradually decreased as the temperature rose. The average number of eggs laid by a single female at 27 ℃ was the highest, reaching 21.12±3.45, and that at 36 ℃ was the lowest (6.8±2.44). Under high temperature treatment (33-45 ℃), the larval pupation rate, pupal emergence rate, and adult survival rate decreased with the increase in temperature, and the treatment time did not exert significant effect. The 5-day-old adults showed stronger tolerance to high temperatures than those of other days, and 17.5% of the adults survived after being treated at 45 ℃ for 90 min. The survival rate of insects at each stage reached more than 85% at 33-39 ℃. The developmental periods of adults and pupae shortened as the temperature increased. At the same temperature, the developmental periods of adults and pupae shortened with the extension of treatment time. 【Conclusion】 The larval survival rate is the highest at 27 ℃, and the pupal survival rate is the highest at 30 ℃, indicating that 27-30 ℃ is a suitable temperature range for the survival of L. botrana. The critical high temperature range is 42-45 ℃. The short-term high temperature treatment will adversely affect the growth, development, and survival ofL. botrana. However,L. botrana has strong tolerance to high temperatures, and high temperature treatment will accelerate its growth to a certain extent.