福建省草地贪夜蛾对5种常用杀虫剂的抗药性
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福建省自然科学基金项目(2022J01459、2021J05088); 福建省公益类科技计划项目(2021R1024008、2020R1024007); 福建省“5511”协同创新工程项目(XTCXGC2021011); 福建省农业科学院科技创新团队(CXTD2021002-1); 福建省农业科学院科技自由探索项目(ZYTS202211); 中央引导地方科技发展资金项目(2023L3059)


Resistance monitoring of the fall armyworm, Spodoptera frugiperda to five commonly used insecticides in Fujian Province
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    摘要:

    【目的】 明确草地贪夜蛾对5种常用杀虫剂的相对敏感基线及其在福建省的抗药性水平,并评价其低龄幼虫对这些药剂的敏感性。【方法】 以浸叶法测定5种杀虫剂对草地贪夜蛾相对敏感种群1、2龄幼虫的毒力,监测福建省3个不同地区草地贪夜蛾田间种群的抗药性水平;以LC50的95%置信限不重叠作为标准,判断不同种群的同一龄期幼虫或同一种群的1、2龄幼虫对同一种药剂敏感性差异显著性。【结果】 5种杀虫剂对草地贪夜蛾相对敏感种群幼虫的毒力大小顺序依次为甲维盐>乙基多杀菌素>氯虫苯甲酰胺>虱螨脲>茚虫威,同一药剂对1龄幼虫的毒力大于2龄,其中,甲维盐、乙基多杀菌素和虱螨脲对1龄幼虫毒力测试的χ2值小于2龄,P值更大,虽然氯虫苯甲酰胺和茚虫威对1龄幼虫毒力测试的χ2值略大于2龄,但P值均大于0.9190;福建3个地区的草地贪夜蛾种群对甲维盐和茚虫威的抗性水平处于敏感性下降至低水平抗性之间,对氯虫苯甲酰胺已产生低水平抗性,对乙基多杀菌素属于敏感性水平下降阶段,而对虱螨脲仍属于敏感水平。【结论】 福建省草地贪夜蛾种群对虱螨脲保持敏感水平,对其他4种常用药剂总体上处于敏感性下降至低水平抗性阶段;1、2龄幼虫均适合作为草地贪夜蛾毒力测定的靶标试虫,但相较于2龄幼虫,12 h内孵化且未喂食物的1龄幼虫适合性更好。

    Abstract:

    【Aim】 To clarify the relatively sensitive baselines and resistance levels of Spodoptera frugiperda to five commonly used insecticides in Fujian Province, and evaluate the sensitivity of young larvae of S. frugiperda to these insecticides. 【Method】 The toxicities of the five commonly used insecticides towards the 1st and 2nd instar larvae of the relatively sensitive population of S. frugiperda were determined using the leaf dipping method. Monitoring resistance of S. frugiperda populations from three different regions to these insecticides was performed. The 95% confidence limits of LC50 values that did not overlap were used as the criterion for judging the significant differences in sensitivity of the same instar larvae from different geographical populations or between the 1st and 2nd instar larvae of the same population to the same insecticide. 【Result】 The toxicities of the five insecticides to the larvae of the relatively sensitive population of S. frugiperda were as follows: emamectin benzoate>spinetoram>chlorantraniliprole>lufenuron>indoxacarb. The insecticides exhibited higher toxicity to the 1st instar larvae than to the 2nd instar. The data obtained in the toxicity testing of emamectin benzoate, spinetoram, and lufenuron on the 1st instar larvae of S. frugiperda showed a smaller chi-square value and larger P-value than that for the 2nd instar larvae, whereas in the toxicity testing of chlorantraniliprole and indoxacarb, the 1st instar larvae showed slightly larger chi-square value than that for the 2nd instar larvae, but their P-values were both above 0.9190. The results indicated that the degree of resistance of S. frugiperda field populations from the three different regions to emamectin and indoxacarb was at the stage of decreased sensitivity to low-level resistance in Fujian, and that the pest had developed low resistance to chlorantraniliprole. The degree of resistance of the three different geographical populations to spinetoram was at the stage of decreased sensitivity, however all populations maintained sensitivity to lufenuron. 【Conclusion】 The population of S. frugiperda remained sensitive to lufenuron in Fujian Province. Overall, the resistance degree of the pest was at a stage of decreased sensitivity or low-level resistance to the other four common insecticides. Both the 1st and 2nd instar larvae are suitable for the toxicity testing of insecticides against S. frugiperda; however, the 1st instar larvae that hatched within 12 h without feeding are more suitable than the 2nd instar larvae.

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邱良妙,陈秀琴,卢学松,刘必炮,刘其全.福建省草地贪夜蛾对5种常用杀虫剂的抗药性[J].生物安全学报中文版,2025,34(2):155-161,179

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  • 收稿日期:2024-04-09
  • 最后修改日期:2024-06-20
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  • 在线发布日期: 2025-05-16
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