【Aim】 Genetically modified (GM) insect-resistant maize, Zheda Ruifeng 8, is a new type of insect-resistant maize developed by Hangzhou Ruifeng Biotechnology Co. Ltd., and it was approved for an agricultural genetically modified organism safety certificate (for production and application) in 2021. This study aimed to design specific amplification primers and TaqMan probes for the transgenic insect-resistant maize Zheda Ruifeng 8 and to quantitatively detect its transgenic components, thereby providing a basis for safety supervision by regulatory authorities. 【Method】 Through a series of experiments, including system optimization, accuracy and precision testing, and robustness testing, specific primers and probes were designed to quantitatively detect the transgenic components of Zheda Ruifeng 8. 【Result】 The data showed that the method can specifically and quantitatively detect the transgenic components of Zheda Ruifeng 8. Positive samples with a copy number ratio of >0.1% (20 copies) were accurately quantified, and positive samples with a copy number ratio of 0.05% (ten copies) were specifically detected. 【Conclusion】 The findings of this study provide effective support for safety supervision by regulatory authorities, thereby indicating that the developed method can be used for the quantitative detection and safety supervision of Zheda Ruifeng 8.