Abstract:【Aim】 Relying on nature-based solutions, screen replacement control plants and their combinations of ecological, economic, and wide-ranging adaptability offer technical measures for controlling the spread of the malignant alien weed Solanum rostratum, which is invading the interlacing region of grazing and agriculture in northern China. 【Method】 This work employed pot experiments with varied soils in several locales to screen the target replacement control plant using a two-factor multi-block asymmetric substitution experiment. After S. rostratum was planted alone or in combination with pastures, the chlorophyll value, aboveground and belowground biomasses, and individual plant biomass of S. rostratum were measured to identify the best replacement control plant, which was then confirmed by field plot experiments. 【Result】 In the cinnamon soils (S1, S2) treatment group, the best replacement control plants are Leymus chinensis, Agropyrom cristatum, and Festuca arundinacea; however, their performances were different between the S1 and S2 treatment groups; the biomass inhibition rates of S. rostratum were 92.90%, 92.31%, and 92.31% in the S1 treatment and 56.7%, 78.4%, and 54.9% in the S2 treatment. The best replacement control plants for the castano-cinnamon soil (S3) treatment group were Medicago sativa, L. chinensis, A. cristatum, and F. arundinacea, with a S. rostratum's biomass reduction percentage of 94.20%, 91.95%, 87.35%, and 86.21%, respectively. The best replacement control plant in the treatment of castanozems (S4, S5) was F. arundinacea, which reduced the biomass of S. rostratum by 97.26% to 85.19%. The inhibition rates of S. rostratum by A. cristatum were 88.41% in the S4 treatment, and that of M. sativa was 88.89% in S5. In the chernozems (S6) treatment group, the best replacement control plant was M. sativa, with an S. rostratum biomass reduction percentage of 97.26%. In the alluvial soil treatment group (S7), the best replacement control was L. chinensis. And A. cristatum with S. rostratum biomass reduction percentages of 94.57% and 85.27% respectively. The results of the plot experiment showed that M. sativa+L. chinensis, M. sativa+L. chinensis, and F. arundinacea had the best inhibitory effects on S. rostratum, with S. rostratum reducing percentages of 91.78% and 91.2%, respectively, compared to the control group (S. rostratum planted alone). 【Conclusion】 Among different soil treatment groups, M. sativa, F. arundinacea, and L. chinensis had the best comprehensive replacement effect. In treatments with higher organic matter contents, F. arundinacea and L. chinensis showed better competitive inhibition of S. rostratum. M. sativa had a stronger inhibitory effect on S. rostratum in the soil treatment group with lower organic matter, M. sativa had a better inhibitory effect on S. rostratum. The combination of M. sativa and L. chinensis and the combination of M. sativa, L. chinensis, and F. arundinacea had the best effect on controlling S. rostratum in the Horqin Steppe of Baicheng City, Jilin Province. These combinations are inexpensive and long-term planting can be used to rebuild the ecological environment and obtain economic benefits.