基于COⅠ基因片段的石榴螟遗传多样性
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高校基本科研业务费科研项目(XJEDU2022Z006、XJEDU2023P107)


Genetic diversity analysis of Ectomyelois ceratoniae (Lepidoptera: Pyralidae) based on COⅠ gene sequences
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    摘要:

    【目的】石榴螟是一种重要的检疫性有害生物,但我国尚未有分布记录。该虫寄主范围广,危害性大,而该虫的寄主在我国分布广泛,因此,开展基于线粒体COⅠ基因的石榴螟遗传多样性和系统发育分析研究,有助于认识世界种群的遗传多样性及各国种群的遗传关系,对进出口商品检疫监管具有重要指导意义。【方法】收集世界各个国家和地区公开的石榴螟种群信息,应用石榴螟线粒体COⅠ基因序列,进行遗传多样性、系统发育、遗传分化情况分析。【结果】结合截获样品扩增的COⅠ基因测序后的序列进行遗传多样性分析发现,世界范围内石榴螟种群存在18个单倍型。单倍型Hap_2为分布最广泛的单倍型,分布于突尼斯、意大利、马耳他、希腊、伊朗、澳大利亚、美国,本次截获的样本均为此单倍型。在多个国家分布的还有Hap_4、Hap_8、Hap_11、Hap_15 4种单倍型。系统发育分析表明,世界范围内12个国家和地区的石榴螟种群聚为2支,澳大利亚2个地区的种群出现了分化。【结论】本研究明确了世界范围内的石榴螟具有高度遗传同质性,仅澳大利亚的2个单倍型出现分化;该物种的变异主要来源于种群之间;COⅠ基因显示出高单倍型多样性,世界范围内的种群反应出较高程度的遗传多样性和中高度水平的遗传分化。

    Abstract:

    【Aim】 Ectomyelois ceratoniae is a moth pest that damages pomegranate trees, but it has not yet invaded China. This pest has a wide host range and is highly harmful; its hosts are widely distributed in China. Therefore, conducting genetic diversity and phylogenetic analyses of pomegranate moths based on the mitochondrial COⅠ gene can clarify the genetic diversity of world populations and the genetic relationships among populations in different countries, which is important as a guide for the quarantine and supervision of import and export commodities. 【Method】 Information on pomegranate moth populations publicly available in various countries and regions around the world was collected, and the mitochondrial COI gene sequence of the pomegranate moth was used for the analysis of genetic diversity, phylogeny, and genetic differentiation. 【Result】 Combined with the analysis of the genetic diversity of COI gene sequences amplified from intercepted samples, it was observed that 18 haplotypes of pomegranate moth populations were reported worldwide. Hap_2 was the most widely distributed haplotype in Tunisia, Italy, Malta, Greece, Iran, Australia, and the United States, and all samples intercepted in this study belonged to this haplotype. In addition, four haplotypes, Hap_4, Hap_8, Hap_11, and Hap_15, were distributed across multiple countries. Phylogenetic analysis grouped the pomegranate moth populations in 12 countries and regions worldwide into two branches, and the populations in the two areas of Australia were differentiated. 【Conclusion】 This study shows that the pomegranate moths invading various countries worldwide have high genetic homogeneity, with only two haplotypes in Australia showing differentiation. Variation in this species mainly comes from between populations; the COI gene shows a high haplotype diversity, and the populations worldwide show a high degree of genetic diversity and a moderate to high level of genetic differentiation.

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刘东,徐新龙,努尔艾孜孜·努尔麦麦,张秀英,陈光辉.基于COⅠ基因片段的石榴螟遗传多样性[J].生物安全学报中文版,2024,33(4):327-335

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  • 收稿日期:2023-11-27
  • 最后修改日期:2024-03-30
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  • 在线发布日期: 2024-11-15
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