【Aim】 This study aimed to understand the sublethal effect of chlorantraniliprole on rice water weevil (Lissorhoptrus oryzophilus Kusehel) and to provide reference for the scientific and efficient control of L. oryzophilus Kusehel using chlorantraniliprole in rice production. 【Method】 Using the overwintering L. oryzophilus adults as test insects, the median lethal concentration (LC₅₀) and sublethal dose ( LC₂₅) of chlorantraniliprole to populations RW-Js, RW-Gy, RW-Mz, RW-Zy, and RW-Lc were determined by measuring the sensitivity of L. oryzophilus to chlorantraniliprole. The effects of sublethal doses of chlorantraniliprole on the activities of various biological enzymes and field development dynamics of Lissorhoptrus oryzophilus were studied using indoor measurements and field investigations, respectively. 【Result】 Significant differences were noted in the sensitivity of different L. oryzophilus populations to chlorantraniliprole, with the LC₅₀ value of RW-Js population being 13.45 times that of RW-Gy population, reaching a moderate resistance level. The synergistic effect of the detoxification enzyme inhibitor PBO on chlorantraniliprole was significantly greater than that of TPP and DEM. The activities of the three detoxifying enzymes in the resistant population, RW-Js, were significantly higher than those in the sensitive population, RW-Gy (P<0.05), with the most significant change observed in multifunctional oxidase (MFO). The activities of several biological enzymes in the L. oryzophilus treated with LC₂₅ dose of chlorantraniliprole increased first and then decreased, and the activity of MFO was significantly higher than that of the control treatment throughout the entire measurement period (P<0.05). The LC₂₅ dose of chlorantraniliprole had a certain degree of impact on the initial, peak, and final stages of L. oryzophilus occurrence in the field. 【Conclusion】 The activity of detoxification enzyme is possibly related to the sensitivity of L. oryzophilus to chlorantraniliprole. MFO is the most important detoxification enzyme in the metabolism of chlorantraniliprole in L. oryzophilus, and plays a major role in the development of resistance. Sublethal doses of chlorantraniliprole can delay the growth and development of L. oryzophilus larvae, pupae, and first-generation adults, shorten their lifespan, and accelerate their extinction in the field.