【Aim】 This study aimed to investigate the inhibitory effects of Pleione bulbocodioides (Franch.) Rolfe ethanol extracts on plant pathogenic fungi and to provide a basis for the development of plant-derived fungicides. 【Method】 The mycelial growth rate method was used to investigate the inhibitory activity of the extract against 15 plant pathogenic fungi. The effects of the extract on the mycelial dry weight, cell membrane, catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activities of pathogenic fungi were further studied using the fungus Fusarium oxysporumf as the test strain. 【Result】 The P. bulbocodioides extract showed significant inhibitory effects on Phytophthora capsici, Fusarium oxysporum, Botrytis cinerea, and Phytophthora cryptogea, with EC₅₀ values of 0.849, 0.782, 0.813, and 1.161 mg·mL^-1, respectively. The mycelial dry weight of F. oxysporumf decreased with increasing extract concentrations. The malondialdehyde content of the cell membrane and relative conductivity increased. The activities of CAT, POD, and SOD, three protective enzymes in mycelia, increased. 【Conclusion】 Extracts from the pseudobulb of P. bulbocodioides had good inhibitory activity against plant pathogenic fungi. This inhibitory effect may be related to its interference with mycelial growth and damage to the normal functions of the mycelial cell membrane.