【Aim】 To provide a scientific basis for the prevention and control of alien fish invasion and aquatic ecological environment protection in Guangxi, risk assessment and suitable area prediction of alien fish invasion in the inland waters of Guangxi were performed. 【Method】 The alien fish invasion risk assessment system and aquatic biological invasion capability screening system (aquatic species invasiveness screening kit, AS-ISK) were used to screen fish species at risk of invading the inland waters of Guangxi, and the maximum entropy model (maximum entropy, MaxEnt) was used to predict fish at high risk of invasion in the inland waters of Guangxi. 【Result】 Eighteen species of alien fish were naturally distributed in the inland waters of Guangxi. Thirteen fish species, including Clarias gariepinus, Oreochromis niloticus, Oreochromis mossambicus, Oreochromis aureus, Pterygoplichthtys pardails, Coptodon zillii, Micropterus salmoides, Ictalurus punctatus, Piaractus brachypomus, Labeo rohita, Prochilodus lineatus, Cirrhinus mrigala, and Gambusia affinis, had high invasion risk, while Tinca mosaics and Neosalanx taihuensis had medium invasion risk. The prediction results of fish with high invasion risk showed that Qianjiang River, Yujiang River, and Nanliu River were the most vulnerable to fish invasion. 【Conclusion】 Both medium- and high-risk fish should be monitored, and continuous monitoring and early screening should be conducted in waters with a high risk of invasion.