In 2019, plants suspected to be Cyclachaena xanthiifolia (Nutt.) Fresen. were found during the field trek of invasive pests in Tacheng, Xinjiang in 2019. The traditional morphological identification method was used to identify the root, stem, leaf, flower, fruit and other external morphological characteristics of the plant. Then specific primers (IXR, IXP and IXM respectively) were designed for polymerase chain reaction (PCR) amplification and sequencing based on the Rps16、psbA and matK gene sequences. The morphological and DNA molecular identification showed that the wild weed from Tacheng area was C. xanthiifolia, and the similarity between the plant gene sequencing fragment and the psbA and matK gene sequences of C. xanthiifolia was up to 98%, which are one of the entry plant quarantine pests. In this study, a rapid detection method for false celandine was established, which can provide reference for the inspection and quarantine and effective prevention and control of false celandine in Xinjiang region.