Phytoseiidae belongs to the superfamily Ascoidea. Most phytoseiid mites are important natural enemies of harmful mites and pests and have important application value in agricultural production. The mitochondrial genome has a unique signature that has attracted extensive attention from biologists. This paper reviews the structure, non-coding regions, base composition, gene rearrangement, and tRNA characteristics of the mitochondrial genome of Phytoseiidae. Its features are as follows: (1) the largest mitochondrial genome of Chelicerata was found in the Phytoseiidae; (2) the AT content of the non-coding regions of the mitochondrial genomes of the Phytoseiidae varies greatly; coding regions are similar; (3) Phytoseiidae mitochondrial genomes have undergone varying degrees of gene rearrangement; (4) the tRNA secondary structure of some species of Phytoseiidae has been truncated and base mismatched; and some phytoseiid mites have anticodon mutations. In future studies, we will increase the sample of the mitochondrial genome determination in key groups of Phytoseiidae and further analyze the reasons for the large number of gene rearrangements in Phytoseiidae to obtain the real evolutionary process of Phytoseiidae species.