【Aim】 Scolytinae was found on Pinus thunbergii in Laoshan, Qingdao City, Shandong Province. In this study, the collected Scolytinae were identified to identify the species and to better investigate the biodiversity and carry out forest trunk borers and control to provide a basis. 【Method】 The morphological characteristics of the larvae, pupae, and adults Scolytinae collected from the P. thunbergii in Qingdao were observed, photographed, and compared. The genomic DNA was extracted, and the sequences of the mitochondrial cytochrome oxidase subunit Ⅰ ( COⅠ ) were amplified and sequenced using general primers of the subfamily. The sequences were compared with the existing sequences in the NCBI database. 【Result】 Based on both morphological and molecular biological identification, this collection was clearly identified as a Cyrtogenius luteus and was determined to have colonised Qingdao. Adults were yellow of C. luteus and pronotum with similar transverse notches. Elytron with uneven grooves and with sparse pili around body. Females have more hair than males of head. The phylogenetic tree (ML tree) based on the COⅠ gene sequences showed that the sequences from Qingdao samples and Italy samples were clustered into one branch with 100% confidence. 【Conclusion】 The results of this study provide basic data for the rapid DNA identification of the Scolytinae, as well as a theoretical basis for the monitoring and control of the trunk borers on P. thunbergii.