[Aim] Systematic investigation of the biological pesticide activity of ethanol extract from the shell of Gleditsia sinensis Lam (Leguminous Gleditsia) extracts could contribute to the utilization of G. sinensis resources and the development of plant-derived pesticides.[Method] Laboratory and field molluscicidal activity of G. sinensis extract ethanol extract from the shell of G. sinensis. was determined via immersion tests.[Result] G. sinensis extract showed significant molluscicidal activity against Pomacea canaliculata, with an LC₅₀ of 40.56 mg·L^-1 for young snails and 109.83 mg·L^-1 for adult snails after 72 h. The field experiment demonstrated that the egg mass was reduced by 100.00% (the adults were unable to lay eggs) and the control effect was (99.12±1.26)% after 40 g·m^-2 of G. sinensis extract was applied for 7 days.[Conclusion] G. sinensis extract had remarkable molluscicidal activity and a good control effect on P. canaliculata. Consequently, it is a potential botanical molluscicide.