[Aim] Paracarophenax alternatus is the first species reported to parasitize and kill Monochamus alternatus eggs. Studies on acclimation effects in P. alternatus resulting in extreme temperature tolerance and low-temperature storage may help assess the potential geographical distribution or application area, and provide a basis for insights into large-scale transportation and storage durability of this mite. [Method] After acclimatisati on at different temperatures, survival rates of P. alternatus were recorded following exposure to extreme temperatures. The number of adult mites released from the expanding abdomen of P. alternatus and survival rates of adult female mites were compared under different low-temperature conditions. [Result] Survival rates of adult female mites exposed to high temperatures increased 1.26-fold after acclimatisati on at 30 ℃ for 36 h. Survival rates of adult females exposed to extremely low temperatures significantly increased (2.08-fold and 2.13-fold) after acclimatisati on at 10 ℃ for 96 h and 120 h. Low-temperature storage effects were assessed using the expanding abdomen as a storage object. The number of emerging mites gradually and significantly decreased with increasing storage time. When female adult mites were used as storage objects, the survival rates of adult females were 65.10% at 10 ℃ for 30 d and 84.21% at 4 ℃ for 30 d. [Conclusion] P. alternatus showed high temperature adaptability. Short-term temperature acclimatisati on can markedly increase its tolerance to extreme temperatures. The expanding abdomen is suitable for short-term storage, and adult females are suitable for medium- and long-term storage.