[Aim] To study the antagonistic effects of strains from the rhizosphere soil of Verticillium wilt-resistant cotton (Hai 7124) against Verticillium dahliae and Panagrellus redivivus, and to provide a model for the biological control of Verticillium wilt and P. redivivus. [Method] The strains were identified through microscopic examination, sugar fermentation experiment, carbon source assimilation experiment, 26S rRNA sequencing, morphology, physiological and biochemical characteristics, and phylogenetic analyses. Masculine screening, Congo red staining, plate confrontation experiments, pot experiments, and flat bioassay experiments were used to test the enzyme production activity and inhibition of V. dahliae and nematicidal activity. [Result] Yeast strain YIN9 was screened from a large number of rhizosphere soils of Verticillium wilt-resistant cotton (Hai 7124). The results showed that the YIN9 strain belonged to Candida. The plate confrontation experiment showed that the inhibition rate of YIN9 on V. dahliae was up to 59%. After co-cultivating the sterile fermentation filtrate of strain YIN9 with V. dahliae spores for 12 h, the microscopic examination found that in the experimental group treated with strain YIN9, most of the spores of V. dahliae could not germinate normally. Pot experiments indicated that the average control effect of strain YIN9 on cotton Verticillium wilt was 60.02%, which could significantly reduce the incidence and disease index of Verticillium wilt in susceptible cotton. Moreover, compared with other yeast strains isolated from rhizosphere soil of Verticillium wilt-resistant cotton, the strain YIN9 showed comparatively high nematicidal activity: the average mortality of nematodes was 90% and 100% after 48 and 60 h of treatment with YIN9, respectively. After boiling the fermentation broth of strain YIN9, both its nematicidal and inhibitory activities against V. dahliae decreased sharply. Further experiments showed that this strain had high protease and cellulase activities. [Conclusion] It was speculated that the biocontrol factor in YIN9 might be a thermally unstable substance, which showed high nematicidal activity and could significantly improve the resistance of susceptible cotton to Verticillium wilt.