10种农业化学品对柑橘内生菌枯草芽孢杆菌L1-21的影响
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科技部重点研发计划项目(2018YFD0201500); 国家自然科学基金(32050410307)


Effect of ten agrochemicals on the indigenous endophyte Bacillus subtilis L1-21 in citrus leaves
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    [目的] 明确农业化学品对柑橘内生菌的影响,指导柑橘合理用药。[方法] 采用平板菌落计数法,测定了6种杀虫剂、3种杀菌剂和微肥EDTA-Cu对生防菌株枯草芽孢杆菌L1-21的相容性及对柑橘植株内生菌的抑制作用。[结果] 56.00 mg·L-1吡虫啉、121.80 mg·L-1虫螨腈、4.00 mg·L-1甲氨基阿维菌素、50.00 mg·L-1噻虫嗪、45.60 mg·L-1氟氯氰菊酯、800.00 mg·L-1辛硫磷、402.00 mg·L-1喹啉铜、3728.00 mg·L-1硫磺、620.60 mg·L-1氧化亚铜和321.00 mg·L-1 EDTA-Cu在平板上对L1-21的抑制率均达100.00%。2倍剂量处理离体叶片24 h后,除8.00 mg·L-1甲氨基阿维菌素的抑制率(16.84%)显著低于其他5种杀虫剂外,其他5种杀虫剂对L1-21的抑制率均无显著差异。2倍剂量处理柑橘活体叶片24 h后,8.00 mg·L-1甲氨基阿维菌素对柑橘叶片内的L1-21抑制作用(4.23%)不明显,显著低于其他5种杀虫剂和3种杀菌剂与微肥EDTA-Cu;对内生菌总量的抑制率以8.00 mg·L-1甲氨基阿维菌素(6.78%)最低,其他制品达46.90%~70.06%,且差异未达显著水平。[结论] 10种农业化学品在平板上均可抑制L1-21,抑制率的大小与其浓度有关。2倍田间登记使用量处理时,10种农业化学品均可影响L1-21在柑橘叶片上的定殖及降低柑橘内生细菌总量。因此,田间施用化学品对柑橘内生菌的负面影响不可忽视。

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    [Aim] The present study assessed the effects of agrochemicals on indigenous citrus endophytes. [Method] The compatibility of six pesticides, three bactericides, and one micro-element fertilizer with the endophytic strain Bacillus subtilis L1-21 was tested. [Result] Citrus endophyte suppression ability was monitored through population dynamics on plates and after spraying on citrus leaves. The results suggested that 56.00 mg·L-1 imidacloprid, 121.80 mg·L-1 chlorfenapyr, 4.00 mg·L-1 methylaminoabamectin, 50.00 mg·L-1 thiamethoxam, 45.60 mg·L-1 cyfluthrim, 800.00 mg·L-1 phoxim, 402.00 mg·L-1 oxine-copper, 3728.00 mg·L-1 sulphur, 620.60 mg·L-1 cuprous oxide, and 321.00 mg·L-1 EDTA-Cu completely suppressed the endophyte L1-21 growth on plates . The 2-fold treatment on detached leaves after 24 h suggested an inhibition rate of methylaminoabamectin 8.00 mg·L-1 (16.84%) that was significantly lower than the five pesticides tested. However, no significant difference was found in the inhibition rate by these five pesticides on endophyte L1-21. In vivo results showed that the inhibition rate caused by 8.00 mg·L-1 of methylaminoabamectin on L1-21 inside citrus leaves (4.23%) was not serious, and lower than the ones caused by the five pesticides, three bactericides and micro-element EDTA-Cu. The lowest inhibition rate on citrus endophytes was recorded for 8.00 mg·L-1 methylamino-abamectin (6.78%), and it was 46.90%-70.06% for other agrochemicals with no significant difference among them. [Conclusion] The ten examined agrochemicals can inhibit endophyte L1-21 on plates in a concentration-dependent manner. In addition, these agrochemicals directly affect endophyte L1-21 in citrus leaves, resulting in a reduced number of endophytes, and the adverse effect of these agrochemicals on citrus endophytes cannot be neglected.

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李咏梅,蒋佳容,ShahzadMUNIR,何鹏飞,吴毅歆,谢金思,潘祖贤,何鹏搏,何月秋.10种农业化学品对柑橘内生菌枯草芽孢杆菌L1-21的影响[J].生物安全学报中文版,2022,31(1):34-40

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  • 收稿日期:2021-06-22
  • 最后修改日期:2021-07-06
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  • 在线发布日期: 2022-03-18
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