[Aim] To accurately evaluate the resistance of banana varieties to Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). [Method] Foc TR4 was inoculated into different resistant banana varieties using the dip-root method and two kinds of root filling methods. The severity of banana disease after inoculation was analyzed to compare the screening evaluation method. The resistance levels of the seven banana varieties at the seedling and field stages were dassessed. [Result] It is more efficient and feasible to evaluate the resistance of banana seedling using the suspension perfusion method with PDB medium containing a Foc TR4 spore concentration of 2×10⁵ spores·mL^-1. Based on the results of resistance evaluation at the seedling and field stages, the resistance of seven banana varieties from high to low was as follows: Nantianhuang > Hongyan 3 > Hongyan 5 > Hongyan 2 > Dianjiao 1 > Baxijiao > Hongyan 1. [Conclusion] Under the control condition of Nantianhuang as high resistant variety and Baxijiao as high sensitive variety. The resistance evaluation of Hongyan 3 and Hongyan 5 was medium to strong, Hongyan 2 reached the level of medium resistance, Dianjiao 1 is a susceptible variety, Hongyan 1 were highly susceptible.