Plant viruses affect the growth and development of plants. The strength of infectivity and proliferation of plant viruses can vary among plant species. To prevent the spread of plant virus along with imported host, this paper describes basic techniques for obtaining virus-free plants, including meristem tip culture, heat treatment, heat treatment combined with meristem tip, micro-grafting in vitro, chemical treatment combined with meristem tip, and chemotherapy. Virus-free plant techniques were tested at entry port.