[Aim] The grape fruit borer has been listed in the group of most dangerous quarantine species in China. If it invades to China, serious losses to fruit industry will be caused. Chemical control is the major approach for controlling this pest in different countries. Identification of the gene sequences of Cytochrome P450 monooxygenase (P450), carboxylesterase (CarE) and glutathione S-transferase (GST) can provide knowledge for function analyses as well as insecticide resistance monitoring and management of the species.[Method] In this study, the BGISEQ-500 platform was used for the transcriptome sequencing of adult moths.[Result] A total of 44360 unigenes were obtained after data assembly and de-redundancy, and 28065 unigenes were annotated by homologous alignment with sequences in multiple public databases. Most of the unigenes (26388, 59.49%) were annotated in the Nr database. Unigenes of the moth showed the highest homology (24.11%) with genes of Helicoverpa armigera. Based on sequence analyses, we identified 91 P450 genes, 31 CarE genes and 22 GST genes. Phylogenetic analysis indicated that the P450 genes of the moth were mainly included in the clade 3 and clade 4 and were clustered with CYP genes from other lepidopteran species. The CarE genes acquired are mainly involved in the detoxification of exogenous compounds and in lipid metabolism. The GST genes of the species were included into Delta, Omega, Epsilon, Theta and Microsomal subfamilies of the GST gene family.[Conclusion] This study provides transcriptome information of grape fruit borer and the gene sequences of P450, GST and CarE, which is important for further research of the insecticide resistance and host plant adaptation of the pest.