[Aim] We tested whether the plant Bidens pilosa has an allelopathic effect on dominant weeds in banana plantations. explored the feasibility of planting as an inter-row cover in banana plantations as a weed control measure.[Method] In this study, the allelopathic effects of four concentration gradients of aqueous extracts from B. pilosa on the four dominant weeds in banana plantation were evaluated by the method of determination of biological index in seed germination test. At the same time, the relationship among weeds, banana and soil was studied by pot experiment.[Result] The experiment of Petri dish germination of 4 kinds of weed seeds showed that when the concentration of aqueous extracts from B. pilosa is 0.0125 g·mL^-1, the comprehensive allelopathy effect on Digitaria sanguinalis germinatin was inhibited, but no such effect was observed for seeds of Kyllinga brevifolia, Eleusine indica and Bothriospermum tenellum. At the concentration of 0.025-0.1 g·mL^-1, the germination of all 4 weeds was inhibited; With the increase of application amount of B. pilosa, the effects of weed control and soil fertility increase gradually. When the application rate was 400 g·plant^-1, the control effect on weeds is the best, the control effect calculated by weed number was 78.99%, the control effect calculated by weed fresh weight was 70.60%. Additionally, we registered an increase in banana seedling biomass (19.8%), soil organic carbon (8.7%), soluble nitrogen (10.4%), and available potassium (16.3%).[Conclusion] This study indicated that the application of B. pilosa in banana plantation can have multiple benefits:inhibit the dominant weeds between rows, improve soil fertility, and promote the growth of banana plants.