广东和广西地区柑橘木虱内生细菌的分离鉴定及多样性分析
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国家重点研发计划项目(2018YFD0201500、2017YFD0202000);广东省现代农业产业技术体系创新团队建设项目(2019KJ108)


Identification and diversity analysis of endophytic bacteria from citrus psyllids in Guangdong and Guangxi
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    [目的] 基于广东和广西地区柑橘木虱内生细菌的分离培养及分子鉴定,明确柑橘木虱可培养内生细菌的多样性,为研究内生细菌与柑橘木虱互作、筛选潜在的共生控制候选细菌提供基础。[方法] 采用虫体捣碎和涂布平板法分离柑橘木虱内生细菌,在线BLAST分析菌株的16S rDNA序列,鉴定内生细菌的种属,并构建优势菌群的系统发育进化树和韦恩图。[结果] 共获得114株柑橘木虱内生细菌,归为细菌界的3个门的15个属,其中芽孢杆菌属59株为优势菌群,占分离细菌总数的51.75%;假单胞菌属14株,占分离细菌总数的12.28%;泛菌属10株,占分离细菌总数的8.77%;其他细菌占分离细菌总数的27.19%。[结论] 芽孢杆菌属细菌广泛分布在两广地区的柑橘木虱体内,值得作为潜在的候选细菌进行共生控制柑橘木虱的研究。

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    [Aim] In order to examine the interaction between endophytic bacteria and citrus psyllids and screen for potential symbiotic control candidate bacteria, the citrus psyllids endophytic bacteria were isolated and identified in Guangdong and Guangxi, and the diversity of cultivars that can be cultivated in citrus psyllids was determined. [Method] The endophytic bacteria of citrus psyllids were isolated with the method of insect crushing and flat coating. The 16S rDNA sequences of the strains were analyzed with BLAST online, the species of endophytic bacteria were identified, and the phylogenetic tree and venn diagram of the dominant flora were constructed. [Result] A total of 114 endophytic bacteria were obtained, which were classified into 15 genera and 3 phyla. Among them, 59 strains of Bacillus were classified as the dominant flora, accounting for 51.75% of the total isolated bacteria; 14 strains (12.28% of the total number of isolated bacteria) were from Pseudomonas genus and 10 strains of Pantoea accounted for 8.77% of total number of isolated bacteria. Other bacteria accounted for 27.19% of the total number of isolated bacteria. [Conclusion] Bacillus bacteria are widely distributed in citrus psyllids, and they are worth considering as a potential candidate for the symbiotic control of citrus psyllids.

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宋晓兵,彭埃天,凌金锋,崔一平,程保平,陈霞.广东和广西地区柑橘木虱内生细菌的分离鉴定及多样性分析[J].生物安全学报中文版,2020,29(3):209-216

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  • 收稿日期:2019-12-28
  • 最后修改日期:2020-02-28
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  • 在线发布日期: 2020-09-24
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