[Aim] The TSC1-TSC2 complex:a key signal-integrating node upstream of TOR and downstream of the insulin signaling pathway genes in Leptinotarsa decemlineata was identified by RNAi technology. The aim is to provide more theoretical support for probing the insuline/insuline-like signal transduction pathway of L. decemlineata.[Method] The LdTSC1/2 cDNA sequences were obtained from NCBI (National Center for Biotechnology Information), and the domain structure and phylogeny of these genes were determined by multiple sequence alignment and phylogenetic analysis. The phenotype, metabolism of sugars and fatty acids of dsRNA-feeding larvae was observed, detected and analysed.[Result] The L. decemlineata TSC1/2 was cloned, the LdTSC1/2 showed orthology with the Agrilus planipennis TSC1/2 and Tribolium castaneum, respectively, and the bootstrap values in plylogenetic tree were all 100. By feeding the dsRNA of the 2nd instar larva, the expression level of the target genes was effectively reduced, larvaes mass loss, pupation and emergence rate are significantly reduced, glucose absorption and conversion efficiency is reduced, and triglycerides are decreased, but trehalose content is increased.[Conclusion] By feeding the dsRNA of the 2nd instar larva, the expression level of the target genes was effectively reduced, and the performance of tested insects showed inhibited in carbohydrate and lipid metabolism resulting in fat body and mass loss, and development delay. TSC1/2 regulates the carbohydrate and lipd acid metabolism in L. decemlineata larvae, with the result that leading to development delay.