[Aim] In order to manage the widespread fruit fly pests, the status of natural enemies, as well as the population dynamics of fruit flies and their parasitoids should be known.[Method] In 2014, the species and population of natural enemies of fruit flies at the peach orchard in Xiaohe township, Kunming, Yunnan Province (25°10'55.07″N, 102°47'52.51″E, altitude:2200 m) were monitored using banana bait boxes.[Result] Six species,762 parasitic wasps of fruit flies were collected from April to October at the peach orchard, including Asobara brevicauda, Asobara mesocauda, Leptopilina japonica, Ganaspis brasiliensis, Trichopria drosophilae and Pachycrepoideus vindemiae. Three species of parasitic wasps:A. mesocauda (29.40%), G. brasiliensis (17.85%) and T. drosophilae (47.77%). The population dynamics of these different parasitoids varied seasonally.[Conclusion]According to the population dynamics of fruit flies, the most promising natural enemies are G. brasiliensis and T. drosophilae. Further research on their utilization will be carried out.