[Aim] The alien plant Ageratina adenophora can do serious damage to the environment and agriculture production. Comparative study on soil bacterial community diversity in different invasive regions of A. adenophora greatly enriched the soil microbial hypothesis of invasive plants and provided an important theoretical basis for the prevention and control of A. adenophora.[Method]Phospholipid fatty acid (PLFAs) analysis and 16s rDNA high-throughput sequencing were used to analyze and compare the bacterial communities in the rhizosphere soil of different severely invasive areas of A. adenophora. Based on this, correlation analysis and principal component analysis were used to explore the relationship between soil bacterial community and soil environmental factors.[Result] (1) The ratio of bacterial content in the rhizosphere soil of A. adenophora to the total amount of microorganisms in each sampling area was higher than 60%. The soil bacterial concentration in Y2 was significantly higher than other samples, reaching 123.74 μg·g^-1, but the number of phyla, families and genera detected in this area was lower than other sample areas. (2) At the level of the phylum, the top five phyla with the highest relative abundance in all soil samples are Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, and Verrucomicrobia. In all soil samples, the relative abundance of Proteobacteria is the highest, whose proportion in the bacterial community is more than 30%. At the genus level, the four sample areas have different advantage genera. The dominant groups in Y1 are Pirellula and Bacillus, and the dominant bacteria in Y2 are Lactobacillus, Mycobacterum and Bradyrhizobium, the dominant bacterium in Y3 and Y4 is Sphingomonas and Psedomona respectively. (3) The environmental factors such as soil available potassium, ammonium nitrogen, organic carbon, sucrase, urease and protease have significant correlation with soil bacterial community Alpha diversity index; environmental factors such as soil available potassium, nitrate nitrogen, organic carbon, invertase, urease and protease affect the bacterial community composition in the rhizosphere soil of A. adenophora.[Conclusion] There are differences in the concentration, phylum level and genus level of rhizosphere soil bacteria in different invasive areas of A. adenophora. Soil available potassium, organic matter content, invertase, urease are the main factors affecting soil bacterial community structure.