[Aim] Ageratina adenophora is a noxious invasive weed. After invading China, it spread rapidly to many provinces and cities, which greatly hindered the development of local agriculture, forestry and animal husbandry. We studied the effects of arbuscular mycorrhizal fungi (AMF) on the growth and reproduction of A. adenophora.[Method] Plant height, number of leaves, total leaf area, internode spacing, basal diameter, anthotaxy amount, seed number per anthotaxy, total seed number, 1000 grain weight, and moisture content of A. adenophora inoculated with AMF were measured in a controlled indoor experiment.[Result] The results showed that AMF highly significant affected plant height and ground diameter of A. adenophora (P<0.01). After inoculation with AMF, the number of heads, single inflorescences, and total seeds number of A. adenophora increased by 5.03, 1.51 and 7.64 times, respectively. Seedling length and width, moisture content, germination rate, germinability, germination index of A. adenophora increased by 15.24%, 23.08%, 19.46%, 29.22%, 391.01% and 183.56%, respectively (P<0.05).[Conclusion] The results indicated that inoculation of AMF significantly promoted the growth of A. adenophora. Controlling the number of AMF can effectively inhibit the growth of A. adenophora and affect the flowering and seeding of A. adenophora seeds and the vitality of seeds, which may be important for the rapid spread of A. adenophora.