[Aim] Yunnan Province is very vulnerable to invasive alien pests. It is urgent to understand the current situation of invasive alien pests in the province to achieve better control.[Method] This paper analyses the present situation of invasive alien pests in Yunnan Province their ways of entry and losses caused.[Result] According to the interception records on imported quarantine objects and the batches of goods, the main countries of origina are Myanmar, Laos, Thailand, Vietnam and the Netherlands. According to the classification of quarantine methods, the number of goods inspection is the largest, followed by traveling inspection, and the sum of the two accounts for 99% of the total intercepted batches. Insects are intercepted most frequently, accounting for 70.0% to 73.9% of the total interceptions, followed by weeds, fungi, bacteria, nematodes, viruses, mites and others. The number of grain legumes are captured most, accounting for 46% of the total interceptions.[Conclusion] The corresponding quarantine supervision countermeasures and suggestions were put forward in order to provide reference for port quarantine and reduce the invasion risk of exotic pests.