[Aim] The alien plant Flaveria bidentis can do serious damage to the environment and agricultural production. Understanding the effects of planting growth between F. bidentis and three native plants on the community structure and diversity of arbuscular mycorrhizal fungi (AMF) can further help understand of its impact on soil microorganisms.[Method] An experiment of growth of F. bidentis with one of three native plants, Setaria viridis, Chenopodium album and Melilotus officinalis, was performed in a common garden. Arbuscular mycorrhizal fungi were collected from the rhizosphere soil of F. bidentis or native plants and identified using morphological characteristics. It's used to comparatively study the effects of F. bidentis and three kinds of native plants on the community structure and diversity of AMF when they grew in different pattern.[Result] The AMF species accumulated in the rhizosphere of F. bidentis were related to their concomitant native plant species. The dominant species when F. bidentis was grown with S. viridis were Glomus reticulatum and Rhizophagus intraradices, while when grown with C. album or with M. officinalis, Glomus reticulatum, R. intraradices and Septoglomus constrictum dominated. The AMF species in the mixed treatments of F. bidentis and S. viridis, F. bidentis and M. officinalis were higher than that in the native single treatment and single treatment of F. bidentis, suggesting that the types of AMF changed with the invasion of F. bidentis and the diversity of above-ground plant species. Compared with the three species of native plants, the Important Value of R. intraradices in the rhizosphere of F. bidentis increased in both the single and mixed treatments of F. bidentis, indicating that the presence of F. bidentis is beneficial to the growth and development of R. intraradices.[Conclusion] The presence of F. bidentis can influence the AMF community structure and diversity in its rhizosphere soil. Native plants present in the community and level of invasions can also influence AMF community.