[Aim] Chouioia cunea Yang (Hainan population) is a native parasitoid that attacks pupae of Opisina arenosella. To assess the potential of the wasp massive rearing for biological control, we investigated C. cunea functional response on O. arenosella in laboratory conditions.[Method]We investigated the effects of nutrition (10% honey) and host stage (early, mid and late pupa) on the functional responses based on observation of its parasitic behavior.[Result] Endoscopic observations showed that C. cunea attacked more than one host. The number of hosts parasitized by C. cunea increased with increasing host density. However, the increase rate gradually slowed down when the host density reached a certain level and the functional response of C. cunea fit to Holling Ⅱ. Without nutrition, the theoretical maximum number parasitized by C. cunea was 1.5, 1.4 and 1.2 per day when exposed to early, medium and late pupal stages, respectively. With 10% honey, these values increased to 1.8, 1.7 and 1.3 per day, respectively.[Conclusion] The fitting results from type Holling Ⅱ functional response indicated that the early pupae was the optimal stage for parasitizing. Nutritional supplements increased the capacity of parasitism.