[Aim] We explored whether the introduction of exogenous genes has an impact on the physical properties of maize leaves, which can provide the basic information for the safety evaluation of transgenic maize and reference for the effective utilization of transgenic maize.[Method] Three pairs of maize leaves were sampled including cv. Dabeinong transgenic maize (transgenic CryAb and epsps genes) vs. its untransformed isogenic form as control, cv. IE09S034 transgenic maize (transgenic CryIE gene) vs. its unmanipulated control, and cv. 808-Shuangkang-12-5 transgenic maize (transgenic CryAb/cry2Aj and Gloevo-epsps genes) vs. its unmanipualted control, cv. 808-Ruifeng-1 grown in the experimental field of Yangzhou University Agricultural Experimental Farm. The wax content, chlorophyll content, hair density, depth of vascular bundle, and contents of Si, K, Ca, S, P and Cl of these maize leaves were determined.[Result] Regardless of the cultivar, the wax content, chlorophyll content and vascular bundle depth of transgenic maize leaves were higher than those of the untransformed controls while leaf hair density was lower. The wax content of Dabeinong, IE09S034 and 808 transgenic maize leaves at the tasseling stage were higher than that of the corresponding control by 17.95%, 48.30% and 39.31%, respectively. The vascular bundle depth of IE09S034 and 808 transgenic maize leaves were higher than that of the parental control by 13.70% and 9.21%, also at the tasseling stage, and 10.81% and 14.47% at the anthesis stage, respectively. The leaf chlorophyll content of IE09S034 and 808 transgenic cultivars were higher than that of the parental control by 18.11% and 13.13% at tasseling stage and 16.62% and 14.61% at the anthesis stage, respectively. The hair density of Dabeinong, IE09S034 and 808 transgenic maize leaves at the anthesis stage was lower than that of the corresponding control by 17.70%, 17.43% and 17.78%, respectively. The elemental contents on the leaf surface of three transgenic maize cultivars were all higher than that of the control. At the tassseling stage, Ca and S content in cv. Dabeinong transgenic maize leaves were 64.71% and 61.18% higher than that of the control, the contents of Si, Ca, S, P and Cl in IE09S034 transgenic maize leaves were 110.26%, 16.67%, 44.44%, 46.32% and 20.00% higher than that of the control, the contents of Si Ca, S and P in 808 transgenic maize leaves were 34.78%, 50.52%, 115.47% and 20.41% higher than that of the control, respectively.[Conclusion] The introduction of exogenous genes can induce profound changes in the physical properties of the maize leaves.