[Aim] The increased use of biological insecticides and natural enemies is the general direction of more sustinable crop management, but the safety of biological insecticides on several natural enemies is not known. Therefore, the effects of biological pesticides on natural enemies were studied to provide a basis for a better coordination of these two control methods.[Method] The residual film method and filter paper method were adopted under laboratory conditions to determine the impacts of the recommended field concentrations of 5 biological insecticides (matrine, cineole, rotenone, pyrethrin, potassium laurate) on 4 commercially used natural enemies, the parasitoid Encarsia formosa, the heteropteran predator Orius sauteri, predatory fly Aphidoletes aphidimyza, and the predatory mite Neoseiulus barkeri.[Result] The effects of pyrethrin on E. formosa, O. sauteri, and N. barkeri were severe, reaching total (100%) mortality. The effects of rotenone on E. formosa, A. aphidimyza, and N. barkeri were higher, with mortality of >98.65%. The potassium laurate had small effects on E. formosa, O. sauteri, and A. aphidimyza with <30% mortality. Matrine caused 100% mortality on E. formosa but effects on A. aphidimyza and N. barkeri were small with the mortality of 15.56% and 15.91% respectively. Cineole was lethal to N. barkeri (100% mortality); the effects on O. sauteri, A. aphidimyza were small with the mortality of 15.91% and 6.67% respectively.[Conclusion] Potassium laurate was safe to E. formosa, O. sauteri, and A. aphidimyza; cineole was safe to O. sauteri, A. aphidimyza; matrine was safe to A. aphidimyza, N. barkeri; while the use of rotenone was consistent only with O. sauteri; and pyrethrin only with A. aphidimyza.