[Aim] In order to evaluate the environmental safety of transgenic EPSPS glyphosate-resistant cotton (GRC), developed by China, the adaptability and competitiveness were studied.[Method] The experiment was carried out at the experimental farm of the Cotton Research Institute of Chinese Academy of Agricultural Sciences, Anyang City, Henan Province, China. GRC cultivars were used as test materials along with its recipient material and cv. CRI49 as the control. Vegetative and reproductive growth, yield contributing traits and fiber quality were evaluated and compared under the normal recommended management model.[Result] Compared with the recipient material, GRC showed certain negative effects on the plant height across growth stages, although the differences were not statistically significant. The number of flower buds per plant of GRC was 3.58 fewer than that of the recipient cv. with significant differences at blooming and boll forming stage. The mean boll number was 1.1 more at boll opening stage and the yield was 91.4 kg·ha^-1 in GRC than the recipient material, and fiber quality also improved. The growth factors, yield and fiber quality of GRC were similar to those of cv. CRI49.[Conclusion] The field performance of the transgenic glyphosate-resistant cotton was not inferior to existing non-GM culvtivars.