[Aim] In order to evaluate the safety of transgenic high-methionine soybean ZD91 to the soil component of ecosystems, we studied the impact of transgenic soybean ZD91 on the main organic elements and enzyme activities in the soil.[Method] Rhizosphere soil samples were collected at four different growth stages (seedling, flowering, pod-setting, and maturity) in two consecutive growing seasons, and the effect on soil moisture, pH, a mounts of total C and total N, and invertase and alkaline phosphatase activities were measured under the laboratory conditions.[Result] The results showed that growing transgenic soybean ZD91 had no significant effects on soil moisture, pH, the concentrations of main soil organic elements and enzyme activities in rhizosphere compared to similar parameters in soil under non-transgenic soybean. Invertase and alkaline phosphatase activities showed significant differences in different years and different growth stages.[Conclusion] Transgenic soybean ZD91 is safety for soil ecosystem.