[Aim] With the widespread application of avermectin, the resistance of target organisms is becoming a more and more serious problem. Previous studies suggest that the membrane transporter P-glycoprotein (P-gp) overexpression is essential for resistance of cancer cells to chemotherapeutic drugs. However, insecticide resistance study was limited, partly because suitable commercial insect antibodies were not available.[Method] In this study, we used anti-human antibody for the detection of P-gp in insects. The expression of P-gp was detected by immunoblotting in the avermectin-sensitive and avemectin-resistant strains of Drosophila melanogaster, using mouse anti-human P-gp monoclonal antibody (C219) as the primary antibody.[Result] The expression of P-gp in the avermectin-resistant strain of Drosophila was higher than that in the avermectin-sensitive strain with no obvious nonspecfic bands detected.[Conclusion] The expression level of P-gp in Drosophila may be related to the avermectin resistance. In addition, it is feasible to use anti-human antibody to detect the P-gp level in insects.