[Background] In recent years, the number of seedlings imported by parcel post increased sharply, increasing the potential risk of entry of new pests. Shenzhen port has repeatedly intercepted cyst and root knot nematodes from such shipments.[Method] In October 12, 2015, Shenzhen port found a kind of cyst nematode in the cactus shipments from Japan. Then the nematodes were identified with morphological methods and 28S rDNA sequence amplification and sequencing.[Result] Extracted nematode life forms included females, cysts, juveniles and eggs. Morphological characteristics of the intercepted cyst nematode were basically consistent with Cactodera cacti, and the 28S rDNA of which matched well (99% similarity) with those of the C. cacti deposited in GenBank (accession number DQ328702). According to the morphological and molecular data, the intercepted cyst nematode was confirmed to be the cactus cyst nematode, C. cacti, so far not known to occure in China.[Conclusion and significance] Inspection and quarantine departments need to pay more attention to the parcel post shipments containing plant material. Plant parasitic nematodes identified with the combination of morphological and molecular methods, will yield more accuracy.