[Background] Glufosinate is the second most highly efficient, broad-spectrum, low toxicity and non-selective herbicide after glyphosate. It is expected to become one of the main herbicide in resistant/tolerant transgenic cotton fields in China. When spray glufosinate on transgenic glufosinate-resistant cotton at different growth stages, it is unclear whether it affects the growth and development of resistant/tolerant transgenic cotton.[Method] Glufosinate of 900 g (a.i)·hm^-2 and 1800 g·hm^-2 was applied at the cotyledon, 3~4 true leaf stage, squaring and bloom stages, in the transgenic glufosinate-resistant cotton field, in 2015. Each treatment area was 25.6 m² (8.0 m×3.2 m). The influence of glufosinate to pollen fertility, cotton growth, cotton yields and fiber quality among treatments were compared.[Result] There were no significant effects to growth and development parameters including pollen activity, plant height, stem diameter, yield and fiber quality during different cotton growth stages when exposed to 900 g·hm^-2 glufosinate. Plant height and seed index were affected by high concentration (1800 g·hm^-2) at the cotyledon stage.[Conclusion and significance] It indicated that transgenic glufosinate-resistant cotton have good tolerance when exposed to 900 and 1800 g·hm^-2, which are recommended doses of glufosinate. This study contributed to the assessment of environment safety of transgenic glufosinate-resistant cotton.