[Background] Cylindrocladium parasiticum, the pathogen causing the peanut black rot disease, is listed as an important quarantine pathogen in China, but the pathogen invaded Guangdong Province in 2009. The pathogen is threatening the peanut production. Screening and planting resistant cultivars is an important measure to control the spread of the disease.[Method] Fifteen peanut cultivars widely grown in Guangdong Province were collected, and their resistance to peanut black rot disease was identified and evaluated by artificial inoculation.[Result] The cultivars cv. Zhanhong NO.2 and cv. Zhanyou NO.62 were resistant to peanut black rot disease. Cv.s Zhanyou NO.75, Zhanyou NO.82, Yueyou NO.390, Yueyou NO.410, Zhongkaihua NO.44, Zhongkaihua NO.99 and Shanyouyou NO.1 were moderately resistant, while cv.s Huayu NO.33, Shanyou NO.523, Shanyoufu NO.1, Yueyou NO.18 and Zhanyou NO.53 were susceptible. Cv. Zhongkaihua NO.332 was a highly susceptible cultivar.[Conclusion and significance] The majority of the peanut cultivars grown in Guangdong were at least moderately resistant to black rot disease at present, but some popular cultivars showed high susceptibility. These results could be used to modify the range of layout of peanut cultivars in Guangdong.