[Background] Cucumber green mottle mosaic virus (CGMMV) is one of the most devastating pathogens of cucurbit crops. The virus has invaded more than ten provinces of China and infected crops such as watermelon and cucumber, causing serious economic losses. CGMMV infected watermelon and cucumber in Guangdong Province in 2009, but the resistance of cucumber and other cucurbits was unknomn.[Method] The resistance of 14 cucumber germplasms to a CGMMV isolate was tested using artificial mechanical inoculation and nucleotide sequencing.[Result] The MP nucleotide sequence of CGMMV of Guangdong isolate from the diseased cucurbit samples was 99% similar to other isolates of CGMMV collected in China. All of 14 cucumber germplasms were susceptible to the isolate of CGMMV.[Conclusion and significance] The main cucumber germplasms from Guangdong are not resistant to CGMMV. This result provides a scientific basis for control of CGMMV, and some guidance for cucumber breeding to resist CGMMV in Guangdong.