[Background] The Cotton leaf curl Multan virus(CLCuMuV), transmitted by the whitefly Bemisia tabaci was first reported in Guangdong Province in 2006. Due to the cryptic species structure of the B. tabaci complex, the interacton between the CLCuMuV and the whitefly in southern China is not clear.[Method] We investigated the CLCuMuV infection rates and identified the cryptic species of B. tabaci in Guangzhou and Qingyuan cities in Guangdong Province.[Result] The percentage of China rose plants (Hibiscus rosa-sinensis) infected with CLCuMuV was 53.98%~71.78% in Guangzhou and 38.42%~45.27% in Qingyuan. The whiteflies were a mixture of MEAM1 and AsiaⅡ7, with the latter being in the minority (6.25%~17.71%) in Guangzhou but forming the majority (76.25%~89.17%) in Qingyuan.[Conclusion and significance] With the sick plants range expansion and poison cryptic species of B. tabaci diffusion, CLCuMuV is may spread in large scale, prevention and monitoring work should be developed.