Due to the establishment and operation of the China-ASEAN Free Trade Area, an increasing amount of agricultural goods, especially tropical fruits produced in ASEAN member countries, is being imported to China, increasing the risk of invasion by agricultural pests. To minimse risk but ensure trade with ASEAN countries, an international collaboration project "Early warning and prevention platform for major agricultural alien pests of China-ASEAN" was initiated by the Ministry of Science and Technology of China in 2011. Experts from the Chinese provinces of Guangdong, Hainan and Yunnan, the Guangxi Zhuang Autonomous Region, and the ASEAN countries of Vietnam, Thailand, Philippines, Myanmar, Laos, and Cambodia formed a joint research team, to develop an early-warning and prevention platform for major agricultural alien pests in China-ASEAN region. This included an alien pest database, techniques for rapid identification, port monitoring, quarantine treatments and eradication methods for major agricultural pests. The strategy and technique package for preventing and controlling major agricultural invasive alien pests from ASEAN countries was put forward, which consisted of three defense lines for monitoring and control training and guidance abroad, inspection and quarantine treatment along border and port, and eradication methods.