【Background】 Codling moth （Cydia pomonella) is the most serious fruit tree and quarantine pest in Chinese orchards. The species is host to Wolbachia, Dor subgroup of the A group, which induce cytoplasmic incompatibility. The aim of this study is to find out if the Wolbachia induce cytoplasmic incompatibility to reproductive regulation in C. pomonella. 【Method】 The relative fitness of C. pomonella with active Wolbachia was compared to individuals in which Wolbachia was eliminate by antibiotics. 【Result】 We found no significant differences in development duration, fecundity, hatchability and pupal survival of the F1, and F2 generations between the two groups. Wolbachia infection had no effect on adult life span, the pre-oviposition period, fecundity and female ratio in C. pomonella. Hybridization tests showed that hybrids could lay eggs, and these eggs were normal. 【Conclusion and significance】 Wolbachia in C. pomonella did not induce host cytoplasmic incompatibility.