【Background】 After the Alternaria leaf blight infection of wheat in China in the 1960s, which spread rapidly and caused serious harm to the local economy, it become a constant threat for the production of China′s wheat. 【Method】 We designed specific primers in order to establish a rapid PCR detection method for Alternaria triticina Prasada & Prabhu. The fungal universal primers ITS4/ITS5 were used to amplify the A.triticina by PCR. The PCR products were cloned and sequenced.The bacteria specific primers LJY1 and LJY2 were designed by DNAMAN software. And the reaction system was optimized. 【Results】 PCR reaction was set up: 25 mmol·L-1 MgCl2 2.5 μL, 10 mmol·L-1 dNTP 1.0 μL, 10 μmol·L-1 primers 0.5 μL respectively, the template of DNA was 8 ng, the best annealing temperature was 57.6 ℃. The rapid PCR detection method for the species was established. 【Conclusion and significance】 The testing of the method for detection on the 9 different strains showed that the primer can accurately distinguish wheat leaf blight from other Alternaria fungi. The results for the rapid detection of wheat leaf blight provide an experimental basis, which can effectively used to prevent the bacteria from entering China′s import and export trade of wheat.