【Background】 Soil microbes are an important part for maintaining biological activities. Soil bacteria are diverse, widely distributed and their structural diversity and dynamic distribution are important for the stability of the lithosphere. In this study, the safety of genetically modified crops on soil bacteria was examined. 【Method】 The community structure of the rhizosphere in fields of transgenic cry1Ie maize and its parental non-Bt maize (the control) were measured at different growth stages. This was assessed using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and Phospholipids fatty acid (PLFA) assays. 【Result】 PLFA analysis showed that the bacteria biomass and G+/G- of transgenic cry1Ie maize and maize receptors control of 2012 had no significant difference at the same growth stage, but significant differences were detected for microbial biomass at different following stages. The values of G+/G- were greater than one, suggesting that at each growth stage, microbial biomass of Gram-positive bacteria was greater than Gram-negative bacteria. During the entire growing period, insect-resistant corn with cry1Ie receptor gene control was not a significant different than control corn. Tt was no significant difference in 2013 in the total amount of microbial and bacterial biomass and the ratio of Gram-positive bacteria and gram-negative bacteria between both corn types. The cluster analysis results showed no remarkable difference in DGGE profile of bacteria at the same growth stage. Corn rhizosphere bacterial community structure was relatively stable and similar in both 2012 and 2013. 【Conclusion and significance】 Two years of data showed that insect-resistant cry1Ie transgenic maize had no significant effects on soil bacterial community structure. The study can provide a theoretical basis to evaluate the safety of transgenic maize with cry1Ie gene.