【Background】Genetic engineering has becomes a powerful tool for maize breeding. The transgenic maize has been increasing in popularity. The transgenic maize brings lots of the economic and social benefits, but people pay greater attention to the potential security issues of transgenic maize than in the past. So it is important to evaluate the impact of transgenic maize cultivation on biodiversity. 【Method】Direct observations and pitfall traps were used to investigate the arthropod in transgenic herbicide-resistant maize with EPSPS gene (CC-2) treated with or without herbicide and non-transgenic maize (zheng 58). The community composition and structure and seasonal dynamics of dominant groups were also analyzed in this three treatments. 【Result】Compared with non-transgenic maize, transgenic herbicide-resistant maize with or without herbicide had no significant effects on composition and structure of arthropod community and seasonal dynamics of dominant groups. 【Conclusion and significance】These results suggest that transgenic herbicide-resistant maize (CC-2) has no risk to diversity of arthropods. This study provides basic ecological safety data for releasing of transgenic herbicide-resistant maize with EPSPS gene.