【Background】The fruit pest Drosophila suzukii Matsumura has been introduced into many countries in recent years and has become a major invasive species. D.suzukii has been becoming more and more serious in China during the past several years as well. In fruit production, D.suzukii often cooccurs with other fruit fly species. 【Method】The morphological characteristics of the adult D.suzukii, D.melanogaster, D.hydeiand D.immigrans, which were collected from Weihai of Shandong, Kunming of Yunnan, Zibo of Shandong, and Linyi of Shandong, respectively, were compared. The mitochondrial cytochrome oxidase Ⅰ (mtCOⅠ) gene of the four fruit flies were used to further determine the species. 【Result】Drosophila species can be determined quickly according to the morphological characteristics of the male forewings, male forefeet, female abdomen, and female ovipositor. 【Conclusion and significance】The study shows that S.alterniflora mainly focuses on tiller growth before July, and start to grow quickly after July in Ningde area, which provides a theoretical reference for control of S.alterniflora.