【Background】The red imported fire ant, Solenopsis invicta Buren, is a significant pest that seriously threatens plants and animals, and humans. It was listed as one of the most destructive and aggressive invasive alien species in the world. Since its first appearance in Guangdong Province of mainland China in 2004, it has spread rapidly to other regions. 【Method】In accordance with the guidelines for pest risk analysis of the International Standards for Phytosanitary Measures (ISPM) of FAO, an integrated multi-index evaluation system was performed in the Yunnan Province. It examined five aspects of invasion risk: possibility of introduction, establishment and diffusion, harmful influence and hazard management. 【Result】The risk assessment value for red imported fire ant in Yunnan Province was 2.25. 【Conclusion and significance】Red imported fire ants belong to high risk of harmful pest, and it is essential to implement the corresponding risk management and quarantine in the entry port of Yunnan Province.