【Background】Microsatellite DNA consists of both prokatryotic and eukaryotic genomes. The characteristics include rich polymorphism and easy detection． The technique has been applied in animal and plant genetics and breeding，for the construction for genetic maps. 【Method】Using the 5′ anchored PCR method, microsatellite DNA from 10 important agricultural pests were isolated and analyzed, including Carposina sasakii (Lepidoptera: Carposinidae), Conogethes punctiferalis( Lepidoptera: Pyralidae), Ostrinia nubilalis (Lepidoptera: Pyralidae), Proxenus lepigone (Lepidoptera: Noctuidae), Frankliniella intonsa (Thysanoptera: Thripidae), Thrips hamaiiensis (Thysanoptera: Thripidae),Thrips palmi (Thysanoptera: Thripidae), Drosophila suzukii (Diptera: Drosophilidae), Lissorhoptrus oryzophilus (Coleoptera: Curculionidae) and Phenacoccus solenopsis (Hemiptera: Pseudococcidae). The characteristics of microsatellite DNA in these insects were analyzed. 【Result】The positive clone rates, microsatellite rates, cloning efficiency and redundancy rates of microsatellite DNA among the species were variable. Dinucleotide repeats were the most abundant repeat types (93.2%~100%), while trinucleotide and tetranucleotide were rare. Among the dinucleotide repeats, AC/GT is the most abundant type (89.2%~100%). This is closely related to the anchor primer; The average repeat number within these species was 6.7~8.9 and the highest repeat number (34) was detected in O.nubilalis; The types of microsatellite mainly consist of the perfect type (91.0%~100%). 【Conclusion and significance】The anchored method can be used to reveal the microsatellite DNA in the agricultural pests, and can be used in monitoring and detection.