【Background】Azadirachtin is an important botanical pesticide. Its toxicity on Tirathaba rufivena Walker has been reported to be on contact or to affect the stomach. However the effects of azadirachtin on development and egg production of T.rufivena have never been studied. 【Method】 A bioassay method was used to detect the effects of azadirachtin on larval and pupal development as well as longevity and egg production of T.rufivena at different sub-lethal does. 【Result】 The development of larvae and pupae were delayed, and the longevity of adults was reduced after expoeure to azadirachtin. The number of eggs and their hatchability were also redused. After treated with azadirachtin at sub-lethal does of LC25, LC50 and LC90, the egg production declined by 14.30%, 20.38% and 19.43%, and hatchability reduced 24.24%, 26.89% and 31.60% respectively. 【Conclusion and significance】 Azadirachtin may be used as an effective pesticide on T.rufivena control sincesince it disturbs its development and egg production.