【Background】Lambda-cyhalothrin is a synthetic pyrethroid insecticide, targeting various insects of Lepidoptera, Coleoptera, Heteroptera and mites. Several studies have examined the toxicity of lambda-cyhalothrin on single species but not on several organisms. 【Method】Toxicity of lambda-cyhalothrin on six species (Japanese quail, bee, silkworm, zebrafish, daphnia and earthworm) was tested in laboratory in accordance with the criteria for chemical pesticide environmental safety assessment. 【Result】According to the data obtained from the tests, lambda-cyhalothrin had low toxicity forEisenia fetida (32.3313 mg·kg-1, 14 dLC50), medium toxicity levels forCoturnix japonica(54.4762 mg·kg-1, 7 dLD50) and Daphnia magna (1.2716 mg·L-1, 48 hEC50), high toxicity for Apis mellifera (2.7391 mg·L-1, 48 hLC50), and extremely high toxicity for Bombyx mori and Danio rerio (0.0067 mg·L-1 and 0.0007 mg·L-1, 96 hLC50). 【Conclusion and significance】This paper demonstrated the toxicity of lambda-cyhalothrin on six groups of species.