【Background】The purpose of this research was to study the variation of the stomatal characteristics of Eupatorium catariumin different habitats, and to reveal the growth strategies and adaptation mechanisms in different habitats, so as to provide theoretical information for the control of this invasive plant. 【Method】Optical microscopy was used to observe the stomatal characteristics of E.catarium in collected from eucalyptus forest, cassava field, wasteland and roadsides. 【Result】Light and soil nutrients had a significant influence on the stomatal aperture (the horizontal axis and vertical axis), single stomatal area, total stomatal area, stomatal density and stomatal index. E.catarium in lowlight but high soil water and nutrient conditions had larger stomatal aperture, single and total stomatal areas, but displayed smaller stomatal density and stomatal index, while in high-light but low soil water and nutrient conditions, the opposite was observed: smaller stomatal aperture, single and total stomatal area, but larger stomatal density and stomatal index. 【Conclusion and significance】These stomatal characteristics indicate that E.catarium possesses a certain ecological adaptability to heterogeneous environments.