Abstract:【Backgroud】Bifenthrin is a synthetic organic compound similar to natural pyrethrins, and has been widely applied against agricultural pest during the last decades. Due to its relative stability and long residual life in the environment, its residues have become a serious problem in export fruits, vegetables and tea microbial degradation, due to its speed and environmental safety could be an effective way to deal with the pesticide residues. 【Method】The bacterial strain capable of degrading bifenthrin was isolated from sludge through the enriched culture. It was identified through its morphological, physiological and 16S rDNA sequence analysis. Its degradation characteristics was studied by using single factor tests. 【Result】A bacterial strain BF-3, capable of degrading bifenthrin, was isolated. We identified and characterised a Gram-positive bacterial strain BF-3, belonging to Bacillus cereus genus. The strain showed S growth in minimum salts medium (MSM) containing 100 mg·L-1 bifenthrin, and after 7 days, the bifenthrindegradation reached 87.9%. The single factor tests indicated that the optimal values for pH, temperature and initial inoculum density was pH 7.0, 30 ℃ and D600 nm=1.0, respectively. 【Conclusion and significance】The identified strain BF-3 could efficiently degradate bifenthrin, suggesting its great application potential to eliminate bifenthrin residues in the environment.